Utilization of plasmid pULB113 (RP4::mini-Mu) to construct a linkage map of Erwinia carotovora subsp. chrysanthemi
نویسندگان
چکیده
منابع مشابه
Efficient transformation of Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica.
We used a modified version of the method of Hanahan (D. Hanahan, J. Mol. Biol. 166:557-580, 1983) to transform Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica with the plasmids pBR322, pBR325, and pAT153. The transformation frequency ranged from 1 X 10(2) to 4 X 10(4) colonies per micrograms of plasmid DNA. The nature of these transformants was confirmed by plasmid ana...
متن کاملBiosynthetic utilization of ethanolamine by Erwinia carotovora.
The purified obelin was electrophoresed on 7 % polyacrylamide gels in Tris-glycine buffer, pH 8.3, containing 0.1 mM-EDTA. Gels stained with Coomassie Brilliant Blue showed a single band at the buffer front, which correlated in position with luminescent activity. This activity could be eluted from unstained gel slices by soaking them overnight in 0.1 mM-EDTA-lOmM-Hepes, pH 7.0, at 4°C. Obelin i...
متن کاملPlasmid ColVBtrp maintenance in Erwinia carotovora.
Plasmid ColVBtrp maintenance in Erwinia carotovora cells was followed by measuring kinetics of elimination of plasmid genetic markers and loss of plasmid deoxyribonucleic acid. An E. carotovora mutant stably carrying plasmid ColVBtrp was isolated. Besides stable plasmid maintenance, the mutant showed altered sensitivity to male-specific phage MS2, sensitivity to drugs, and colony morphology.
متن کاملPartial Resistance to Erwinia carotovora subsp. carotovora and Plant Vigour among F1 Hybrids of Zantedeschia Cultivars
The potential of breeding Zantedeschia cultivars for resistance to soft rot caused by Erwinia carotovora subsp. carotovora (syn. Pectobacterium carotovorum subsp. carotovorum) was determined. Hybrids of six cultivars (‘Back Magic’, Galaxy’, Pink Persuasion’, Sensation’, ‘Treasure’ and ‘Florex Gold’) were screened using a non-destructive resistance test (leaf disk test) and scored different leve...
متن کاملRegulation of the expression of prtW::gusA fusions in Erwinia carotovora subsp. carotovora.
Erwinia carotovora subsp. carotovora, a Gram-negative phytopathogenic bacterium, secretes an extracellular metalloprotease, PrtW. Previous results demonstrated that protease activity is necessary for the normal progression of disease symptoms caused by this bacterium. The present study revealed that the prtW gene constitutes an independent transcriptional unit. It is demonstrated that introduct...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1983
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.154.3.1489-1492.1983